Results
| PMID | 8150107 |
| Gene Name | PLA2G2A |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 24 |
| Population details | 24 (15 have patients with endometriosis, 9 do not have endometriosis) |
| Sex | Female |
| Associated genes | PLA2 |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 1994 Apr;61(4):657-62. Sano, M| Morishita, T| Nozaki, M| Yokoyama, M| Watanabe, Y| Nakano, H Department of Gynecology and Obstetrics, Faculty of Medicine, Kyushu University, Fukuoka, Japan. OBJECTIVE: To assess the prostaglandin (PG) production on peritoneal fluid (PF) cells, phospholipase A2 (PLA2) activity of those cells in women with endometriosis was measured and compared with that of women without endometriosis. DESIGN: Prospective clinical controlled study. PATIENTS: Women who underwent laparoscopy and were found either to have endometriosis (n = 15) or not (n = 9) were included in this study. Mononuclear cells obtained from the patients at laparoscopy were immediately separated by a Ficoll-Paque technique, lysed by nitrogen cavitation, and stored at -80 degrees C. INTERVENTIONS: Phospholipase A2 activity was measured by Dole assay using 1-palmitoyl-2-[1-14C] palmitoyl phosphatidyl choline and assessed on a protein basis and a cell number basis. RESULTS: There were at least four measurable kinds of PLA2 activity detected in the cells: two calcium-dependent pH optima 7.0 and 9.0 activities and two calcium-independent pH optima 7.5 and 8.5 activities. A calcium-dependent and pH optima 9.0 activity was the highest, and it was significantly higher in women with endometriosis when compared with those who did not have endometriosis. CONCLUSION: These results indicate that the increase in the PGs in PF with endometriosis may be produced by PF cells in which PLA2 activity is elevated. Mesh Terms: Adult| Ascitic Fluid/*cytology/enzymology| Calcium/pharmacology| Endometriosis/*enzymology| Female| Humans| Hydrogen-Ion Concentration| Kinetics| Macrophages, Peritoneal/enzymology| Phospholipases A/*metabolism| Phospholipases A2| Prospective S |
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